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Title: Section 61-1.11 - Physical containment levels; P1 level

61-1.11 Physical containment levels; P1 level.

(a) Laboratory practices.

(1) Laboratory doors shall be kept closed while experiments are in progress.

(2) Work surfaces shall be decontaminated daily, and immediately following spills of organisms containing recombinant DNA molecules.

(3) All biological wastes shall be decontaminated before disposal. Other contaminated materials, such as glassware, animal cages and laboratory equipment, shall be decontaminated before washing, reuse or disposal.

(4) Mechanical pipetting devices shall be used; pipetting by mouth is prohibited.

(5) Eating, drinking, smoking, and storage of foods are not permitted in the laboratory area in which recombinant DNA materials are handled.

(6) Persons shall wash their hands after handling organisms containing recombinant DNA molecules, and when they leave the laboratory.

(7) Care shall be taken in the conduct of all procedures to minimize the creation of aerosols.

(8) Contaminated materials that are to be decontaminated at a site away from the laboratory shall be placed in a durable leak-proof container, which is closed before removal from the laboratory.

(9) An insect and rodent control program shall be instituted.

(10) The use of laboratory gowns, coats or uniforms is discretionary with the laboratory supervisor.

(11) Use of the hypodermic needle and syringe shall be avoided when alternative methods are available.

(12) The laboratory shall be kept neat and clean.

(b) Containment equipment. Special containment equipment is not required at the P1 level.

(c) Special laboratory design. Special laboratory design is not required at the P1 level.


VOLUME A-1a (Title 10)